The present study was designed to evaluate age-related alterations of spatial memory in rats prenatally exposed to valproic acid (VPA). To induce autism-like animal model, the pregnant rats were intraperitoneally injected 500 mg/kg NaVPA at the gestation day 12.5. Experiments were carried out on three age groups of control and VPA-treated male offspring: prepubertal adolescence (postnatal day 30); adult (6 months) and old (22 months). Spatial memory and learning process was assessed in the Morris water maze (MWM). The effect of treatment on the learning process were analyzed by Two Way Repeated Measures ANOVA. The results of MWM experiments showed that both control and VPA-treated rats met the learning criteria by the end of the training period. When analyzing training phase days 1 and 4 (Tukey Test), animals of all groups showed significantly decreased latency in reaching the hidden platform (P < 0.001, for all groups). During the probe test, which was performed 24 h after task acquisition, the control and VPA-treated adolescent and adult rats as well as VPA treated aged rats showed normal spatial learning and memory abilities in the MWM task, however rats within the control aged group exhibited a retention deficit 24 h after training. In conclusion, we found that spatial memory function in VPA induced rat model of autism is relatively age-resistant and it is our hope that further research will enhance our understanding of aging in an abnormal central nervous system.Supported by the funding from the SRNSFG: Grant #-FR-22-10650