Depression is a common affective disorder that represents a heavy burden on communities. Psychobiotics, the emerging group of probiotics and postbiotics that affect brain function and mental health have shown promising results in alleviating symptoms of depression. Postbiotics, bioactive compounds released by/or produced through the metabolic activity of the microbiota, provide various beneficial effects for the host, including antidepressive ones. This study aimed to evaluate antidepressive potential of postbiotic obtained from GABA-producing B. adolescentis NGB329. We tested whether this postibiotic may prevent or mitigate behavioral and biochemical changes in rats exposed to chronic unpredictable mild stress (CUMS), commonly used animal model of depression. Three-weeks old rats were divided in 3 groups (n=16): (1) control, non-treated rats; (2) rats treated with vehicle (growth medium) and (3) rats treated with postbiotic B. adolescentis NGB329 for 8 weeks. After 4 weeks each group was sub-divided into 2 groups: non-stressed (NS) and stressed (CUMS) for 4 weeks (n=8). Sucrose preference test (SPT), and splash test (ST) were used to test anhedonia, while marble burying test (MBT) and elevated plus maze (EPM) were used to score anxiety in rats. The levels of corticosterone (CORT) and pro-inflammatory cytokines TNF-α, IL-1β and IL-6 in serum were measured using ELISA kits according to the manufacturer’s instruction. Postbiotic B. adolescentis NGB329 have shown protective effects at both behavioral and biochemical level.