This study explores the impact of anodal tDCS on WM deficits induced by stress. We know from previous research that stress conditions lead to deficits in WM performance by reducing WM-related activity in the brain. Specifically, the dorsolateral prefrontal cortex (dlPFC) is heavily involved in WM processes, and the ventromedial prefrontal cortex (vmPFC) plays a crucial role in stress regulation and has been associated with positive emotions during stress recovery. Given these findings, we aimed to determine the most effective stimulation region for alleviating WM deficits caused by psychological stress.Stress induction was achieved using aversive movie clips, while WM assessment was conducted using an n-back task. Employing a sham-controlled mixed design, stress induction served as a within-subject factor, with three stimulation groups (dlPFC, vmPFC, sham) as a between-subject factor, each consisting of 24 participants.The effectiveness of stress induction was validated by observing heightened negative feelings, increased state anxiety, and alterations in physiological stress markers such as Cortisol levels, heart rate, heart rate variability, and Electroencephalogram (EEG) power and connectivity.Behavioral data revealed significant improvements in WM performance, particularly in the 3-back task with vmPFC stimulation, while the sham group demonstrated decreased performance and a significant decrease in Sham for both hits and sensitivity index d’ prime. EEG data analysis corroborated these findings, demonstrating changes in event-related potentials (ERPs) components and event-related spectral perturbations (ERSP). Notably, post-stimulation Cortisol levels exhibited a significant reduction, indicating that brain stimulation effectively mitigated WM deficits by attenuating the impact of stressors.