Emerging evidence suggests that intrauterine exposures to maternal factors such as to maternal pre-pregnancy body mass index (MBMI) impacts the neurodevelopment of the offspring in a region-specific way, possibly contributing to future obesity risk of the offspring. Our study investigates the associations between MBMI and neonatal global white matter integrity in 157 infant-mother dyads from FinnBrain Birth Cohort Study (www.finnbrain.fi). Following TBSS (Tract-based Spatial Statistics) skeletonization, associations between MBMI and mean diffusivity values were assessed using a voxel-wise non-parametric permutation analysis (FSL randomise, 5000 permutations, threshold-free cluster enhancement). We found significant clusters in the left Corpus Callosum that were positively associated with the MBMI (see Figure 1). The findings are in line with the previous literature investigating the impact of obesity on white matter integrity in the adult population. Our results provide valuable insights into the fetal programming of neurodevelopment in obesity. These insights may be potentially useful for early intervention strategies to curb the obesity epidemic. Figure 1. Positive associations between maternal pre-pregnancy BMI and infant left Corpus Callosum mean diffusivity (MD) analysed with voxel-wise general linear model adjusted for infant sex and age from birth in days displayed on the mean MD skeleton mask. Voxels that are significant (p < .05) after threshold-free cluster enhancement correction for multiple comparisons are presented in the figure. The colour bar represents 1 − p values. The figure is in radiological convention.