Brain cognitive performances are typically thought to fully rely on neurons. Yet, recent research has shown that the other major cell type of the brain, the astrocyte, actively influence information processing. Astrocytes are indeed involved in the modulation of neuronal circuit activities underlying behavior, by regulating extracellular concentrations of ion and neurotransmitters. We have previously found that astrocytes control dopamine levels in the prefrontal cortex, thereby modulating synaptic transmission and plasticity. Dopamine release can occur physically, responding to specific stimuli, or tonically, involving spontaneous and discrete liberations. In order to get insight into the role of astrocytes in the phasic or tonic release of dopamine associated with specific behaviors, we imaged dopamine release during prefrontal related behaviors in transgenic mice bearing a conditional deletion of the vesicular monoamine transporter 2 (VMAT2) specifically in astrocytes (aVMTA2cKO mice). Dopamine release was monitored in the prefrontal cortex using miniscope in vivo imaging of the genetically encoded fluorescent sensor GRABDA, expressed on the extracellular neuronal membranes. We have assessed at the anxiety, sociability, motivation and motor components of behaviors using elevated plus maze, 3-chambers and spontaneous social interactions tests. Our results suggest that tonic rather than phasic releases of prefrontal dopamine are regulated by astrocytes.