Synapse formation is critical for the wiring of neural circuits in the developing brain. The composition and function of synapses has predominantly been studied in rodent cells. Here we show a novel protocol to generate human iPSC-derived forebrain-like neurons that show rapid synaptic development. To achieve this, we use an astrocyte feeder layer attached to a complex mix of ECM-components. Neuronal stem cells (NCS) are plated on this layer for maturation into neurons. We use this protocol to provide an initial characterisation of Magi-family synaptic scaffolding proteins in human neurons. Both Magi1 and Magi2 are expressed in the brain, however expression levels of Magi-2 are much higher in neurons than in any other cell type, while Magi-1 is expressed more evenly throughout cell types and tissues. In rodents the synaptic scaffolding protein S-SCAM/Magi-2 plays a critical role in the assembly and maintenance of synapses and interacts with signalling proteins facilitating synapse to nucleus signalling. Increased expression of S-SCAM/Magi-2 in the human brain is associated with schizophrenia. We show that Magi-1 can be found at both inhibitory and excitatory synapses in human neurons and can substitute for the loss of Magi-2. Lastly, we analyse how S-SCAM/Magi-2 levels at the synapse influences gene expression, via synapse to nucleus signalling.