The stress response is a significant precipitating factor in various psychiatric disorders. Numerous neuropsychiatric disorders are characterized by sex differences in the prevalence, type, and severity of symptoms, which include cognitive dysfunctions mainly supported by the prefrontal cortex (PFC). This study aims to evaluate the sexual dimorphic effects of acute stress on PFC synaptic properties, as well as on both the intrinsic and synaptic properties of PFC-basolateral amygdala (BLA) and PFC-PFC projection neurons. Adult male and female mice were exposed to either restraint stress (RS) or maintained in their home cages (NR). Acute brain slices containing the PFC were isolated in order to record evoked field excitatory postsynaptic potentials (fEPSPs) or patch-clamp recordings. In select instances, PFC slices from NR animals were incubated with corticosterone (CORT). Βoth RS and CORT impairs the induction of Long Term Potentiation(LTP) in PFC of male mice only. Additionally, we observed a reduction in PFC inhibition following CORT incubation and RS exposure in female mice only. To further dissect the sexual dimorphic effect of RS, mice were stereotaxically injected with a retrograde fluorescent substance (cholera toxin B) into the PFC or BLA of RS or NR mice, enabling the dissection of cortico-cortical and cortico-amygdalar neuronal properties. Patch-clamp recordings were then performed in fluorescently-labelled neurons of acute PFC slices. No significant baseline differences between males and females were identified in the synaptic properties of PFC->PFC or PFC->BLA neurons. The investigation of RS on the two circuits is currently on-going.