Recent studies have shown that ON-alpha sustained (ON-αs) retinal ganglion cells (RGCs) exhibit distinct distribution patterns within the mouse retina, with a notable prevalence observed in the temporal region. RGCs rely mainly on presynaptic inputs to create meaningful outputs, however the contribution of postsynaptic RGC processing is unknown. Therefore, we compared the intrinsic spiking properties of nasal and temporal RGCs in the wild-type and degenerated mouse retina. Whole-cell patch clamp experiments were performed to measure the responses of temporal and nasal ON-αs RGCs in wild-type and degenerated (rd10, age > P180) mouse retina to light stimulation and intracellular current injections. Immunostaining was performed to reconstruct cell morphology. Similar to the previously reported variations in size along the nasal-temporal axis, prolonged current injections revealed significantly higher maximum sustained firing rates in temporal versus nasal wild-type ON-αs RGCs (168 vs 124Hz). Spiking responses in the rd10 retina were similar to those in the wild-type retina, with the strong nasal-temporal gradient in firing rate being preserved. Morphological analysis revealed that the nasal-temporal size variation was also preserved in the rd10 retina. However, dendritic field diameter and axon initial segment length were significantly reduced in both nasal and temporal rd10 retina. Our findings indicate that the previously reported gradient in cell size of ON-αs RGCs across the mouse retina is paralleled by functional differences. We show that the nasal and temporal cells of the rd10 retina behave similarly to those in wild-type mice implying that rd10 cells preserve their spike generators, despite photoreceptors degeneration.