Navigating space to retrieve uncued rewards at remembered locations depends on intact hippocampal function. Indeed, learning novel reward locations in familiar environments requires synaptic plasticity and lasting reorganization of spatial representations in the CA1 area of the dorsal hippocampus. Such reorganization is absent when learning associations between rewards and non-spatial cues, for which spatial memory is not involved. Thus, the rodent brain selectively adjusts weights of hippocampal inputs, plasticity processes, and the stability of spatial representations, supporting adaptive behavioural responses when switching between spatial and non-spatial strategies. Lesion studies provided evidence that the medial prefrontal cortex is a key upstream structure in orchestrating this network adaptation, yet the underlying mechanisms remain largely unknown. The purpose of our study is to identify and investigate the neuronal circuits and long-range pathways that coordinate the adjustments of hippocampal computations upon strategy switching. We developed a strategy switching task for head-fixed mice that prompts animals to spontaneously and flexibly alternate between spatial and cue-guided search strategies to find and retrieve water rewards in a linear virtual reality corridor. In parallel, we use retrograde and anterograde viral vectors, combining multiple different fluorescent protein tracers, to investigate potential pathways connecting the prelimbic and infralimbic cortices to the CA1 area of the dorsal hippocampus as a downstream target. Combining these approaches provides a viable experimental framework to investigate the circuit mechanisms and neuronal substrates of top-down control by the prefrontal cortex to the dorsal hippocampus during flexible behavioural adaptation. Financial support by grant P37089-B from FWF (Austria).