ePoster

Dendritic imaging of voltage and calcium signals during visual learning paradigm

Anna Jasz, Borbála Kertész, Helén Bartók, Tamás Tompa, Zoltán Szadai, Balázs Rózsa
FENS Forum 2024(2024)
Messe Wien Exhibition & Congress Center, Vienna, Austria

Conference

FENS Forum 2024

Messe Wien Exhibition & Congress Center, Vienna, Austria

Resources

Authors & Affiliations

Anna Jasz, Borbála Kertész, Helén Bartók, Tamás Tompa, Zoltán Szadai, Balázs Rózsa

Abstract

Although dendritic signaling strongly influences sensory processing and learning, the connection between dendritic integration and somatic output is still not fully understood. Our aim is maping the origins of dendritic events during different aspects of visual learning.We used 3D two-photon laser scanning microscopy with acousto-optic deflectors to simultaneously record jRGECO1a (calcium) and JEDI-2p (voltage) signals in the apical dendrites of layer 5 pyramidal cells in the primary visual cortex of mice during visual discrimination. We examined mice with controlled water access participating in visual discrimination tasks, in which half of the trials were randomly rewarded with water. The stimuli consisted of drifting gratings with different orientations to allow the investigation of dendritic events related to visual stimulation. So far we have found that various types of voltage signals appear along the entire dendrite during visual stimulation, and that they seem to be analogous to the calcium signals. This opens up the possibility of determining the origin of the signals detected by the calcium and voltage sensors. Additionally, we have recorded dendritic activity during the training of the animals, during which they had to discriminate between rewarded and non-rewarded visual stimuli. In these measurements, we were able to detect dendritic events related to distinct aspects of the behavioral protocol, such as reward or error signals. Our goal from here is the detailed analysis and characterization of the composition and propagation of these voltage signals.

Unique ID: fens-24/dendritic-imaging-voltage-calcium-signals-cde42b72