The entorhinal cortex lesion is a validated model for studying neuronal, dendritic and spine remodelling of dentate gyrus granule cells after denervation. Our research aims to investigate the effect of the layer-selective denervation of the dentate gyrus on the proliferation and differentiation of neurons in the granule cell layer, as the mechanisms that drive the remodelling due to the loss of input are not yet understood. Therefore, in anaesthetised adult male C57Bl/6 mice, the transection of the perforant pathway was performed, resulting in the layer-specific denervation of dentate granule cells. We injected both the experimental animals and the sham-controls with bromodeoxyuridine (BrdU) and sacrificed them at two time points: after 3 days to investigate the effects of transection on neuron proliferation and after 28 days to explore cell differentiation. We studied the distribution of BrdU, Ki-67, doublecortin (DCX), NeuN and GFAP-positive cells with immunofluorescence labelling and confocal imaging, followed by particle analysis of the labelled cells in ImageJ. The preliminary results show an increase in BrdU- and Ki-67-positive granule cells 3 days after transection in the septal hippocampus compared to sham-operated mice and to the contralateral hippocampus in lesioned mice. By 28 days, there was no difference between BrdU+ and DCX+ dentate granule cells compared to controls in the denervated region. Further experiments will give a deeper insight into neurogenesis in the dentate gyrus of adult mice in vivo and provide a better understanding of the functional recovery of granule cells after entorhinal denervation.