Axolotl, despite being a vertebrate, showcase an astonishing regenerative capacity even in highly complex tissues such as the central nervous system. Most studies in regenerative model systems concentrate solely on molecular or morphological aspects of regeneration, while the examination of functional outcomes in vivo is often overlooked. It is however crucial to assess if morphological regeneration also leads to restoration of function to its pre-injury state.This project addresses axolotl visual behavior through the establishment of a novel assay. Weused a computer-controlled projection system, to present stimuli without direct water contact,to make sure the stimuli remain exclusively visual. Axolotls of varying sizes were subjected to different prey simulations to help us understand their size and shape preference.Furthermore, the influence of various factors on prey hunting behavior, such as sex, ambient lighting, or the treatment of substances that are essential for brain injury studies, was evaluated. This allows a broad categorization of stimulus response events, which lays groundwork for future functional experiments. In subsequent studies, we plan to disturb the axolotl’s visuomotor system by injuring the main visual processing center in the brain, the optic tectum. During the course of regeneration of this brain area, we will explore the extent to which this reestablishes a functional visuomotor circuit by implementing the behavior assay described above.