Astrocytes are crucial mediators of brain function on a multimodal level such as energy metabolism or synapse formation and maturation. They form a vast network in the brain and signal both with each other and with neurons. Calcium is the primary information carrier in astrocytes and can be measured using fluorescent indicators. Therapeutic approaches for cognitive diseases are commonly designed to influence neurons, but neglect astrocytes, despite them accounting for half the mass of the brain.Here we use astrocytic calcium signals and recent advances in analysis to develop an astrocytic compound screening pipeline. Astrocytic calcium signals were measured in mouse hippocampal cocultures of neurons and astrocytes with virally expressed calcium sensors based on GCaMP. Benchmarking was performed with several compounds of known effect. We then verified the pipeline using a compound with unknown effect, the psychedelic LSD, on astrocytic signaling. Using a second neuronal calcium sensor based on RCaMP, we expanded the pipeline to correlate neuronal and astrocytic calcium signals. We demonstrate that neuronal and astrocytic calcium signals are correlated, and astrocytic calcium signals are a potent functional readout for the effect of a compound.In addition to mouse astrocytes and neurons, we are developing cultures of astrocytes and neurons derived from human induced pluripotent stem cells, to investigate potential drug effects on calcium signaling, and predict efficiency in cognitive diseases with greater confidence.