In 2019, a report by world stroke organisation (WSO) states that there are 12.2 million new cases of ischaemic stroke per year with 6.5 million people dying annually. Stroke is the third nationally and second globally highest in terms of mortality and third highest worldwide in terms of Disability Adjusted Life Years (DALYs). Chronic ischaemia, constituting 62% of all stroke cases; and cognitive impairment are reported to be 50% associated in patients younger than 50 years, foisting a huge burden on their families. Post stroke cognitive impairment (PSCI) includes the damages caused to the cognitive domains 3-6 months’ post-stroke. This condition makes the patients incapable of living independently, which without proper treatment, results in high morbidity and mortality. The study of pathogenesis is still scarce and needs an interdisciplinary investigatory approach.Our research involves the induction of global ischaemia in brain through bilateral common carotid artery occlusion (BCCAo) model. We recognise the use of RNA-Seq high-throughput sequencing for identifying and measuring gene expression. The interactome plays an unavoidable role in the pathogenesis of disease. Thus, the protein-protein interaction analysis assesses the significance of disease hubs in the cognitive impairment and neurodegenerative pathways. Our findings highlighted the intriguing role of Akt/Mapk pathway in discrete brain regions to be associated with induction of cognitive deficits. Furthermore, analysing the expression and modulation of the targeted proteins in this selected pathway substantiates their significant role in cognitive impairment, which may be of great importance to patients with PSCI.