The aim of the authors’ research was to assess the effect of moderate‐intensity balance training (BT) on the activity of catalase (CAT), superoxidase dismutase (SOD) and glutathione peroxidase (GPx) in people with Parkinson's disease (PD). Participants and Methods: Volunteers were randomly divided into 2 groups: balance training (BTG, n=10, age 65.3±3.84 years) or control group (CON, n=10, age 66.8±2.38 years). For 12 weeks (3 times/week for 60 minutes), BT was conducted at an intensity of 60-70% of maximum heart rate. Before and after 12 weeks of BT, the activity of antioxidants enzymes was determined in the blood plasma. Results: At baseline the activity of SOD and GPx was similar in compared groups. Prior the start of BT the activity of CAT was higher in CON group. In the BTG group after 12 weeks of BT the activity of CAT and SOD was significantly higher than before (respectively: 132.18±10.55 nmol/min/mL vs. 226.99±57.97 nmol/min/mL , p=0.01; 1.30±0.17 U/mL vs. 1.52±0.18 U/mL, p=0.01) and at the same time, the CAT activity was higher than in the CON (p=0.028). GPx activity did not change in BTG group. CAT, SOD and GPx activity did not change in the CON group. Conclusions: Systematic BT improve the antioxidant defense in people with PD by increasing the activity of CAT and SOD. Acknowledgments: We sincerely thank Participants for their kind cooperation in this study. Research funding. This work was supported by University of Physical Education in Krakow, Poland, grant number: 307/BS/IRK/2023.