Evolution has preserved many molecular pathways, establishing invertebrates as versatile models for exploring the fundamental and universal processes of CNS physiology and pathology. Among them, the pond snail Lymnaea stagnalis serves as an effective model organism for Translational Neuroscience due to its relatively simple CNS and open circulatory system, simplifying the exploration of the complexity of conserved molecular pathways and allowing to investigate how drugs can modulate neuronal functions and behaviors. In this study, we discovered that, despite never encountering natural predators, laboratory-reared snails exhibit anxiety-like behavior, characterized by reduced escape behavior and increased aerial respiration, even without exposure to fish scent. This behavior continued even also in the absence of predation risk. To further characterize this model, we evaluated the effects of alprazolam, one of the most widely prescribed benzodiazepines for generalized anxiety disorder. After standardizing doses and lengths of treatments, we found that exposure to alprazolam 0.1 mM for sixty minutes induced sedative effects at three hours post-treatment and induced anterograde amnesia. The anxiolytic effects emerged six hours after treatment. Differently, when alprazolam was administered for just fifteen minutes, the anxiolytic effects were evident three hours following treatment. This is the first study exploring how a benzodiazepine influences anxiety-like behaviors in response to predator threats in a molluscan model organism. Our results paved the way for the ongoing gene expression and metabolomic analyses in our laboratory aimed at uncovering the conserved mechanisms driving anxiety-like behavior and the impact of anxiolytic medications.