High-intensity white noise (HIWN) pollution from a variety of sources is considered a serious health hazard. HIWN with enough intensity and duration could lead to temporary and permanent hearing threshold level shifts, although its mechanisms are not well understood, and behavior alterations.The auditory system has been extensively researched; learning and memory have not studies. Moreover, the consequences connected to gender are not clearly discussed. You therefore made the decision to contrast and investigate spatial memory in male and female rats.In the present study, we assessed the short-term and long-term spatial memory using a Morris water maze (MWM) for gender differences in rats. Rats were exposed to 100 dB/a hour/30 days.HIWN did affect learning or the components of escape latency, also on Long-term spatial memory. However, compared to the animals in the control group, the animals in the experimental group spent more time in the exploration period-the time spent in different sectors during the probe test.When assessing learning and short-term and long-term memory, the mentioned changes involving time traveled, distance, and speed were pronounced in male rats and significantly inferior to the behavior of the same-age experimental female rats.In particular, it seems that long-term exposure to HIWN impairs an animal's capacity for learning and for both short- and long-term spatial memory.We estimate that HIWN has a more negative effect on male rats' learning process and spatial memory in the MWM test performance example.The study was funded by Shota Rustaveli National Science Foundation. Project #FR-21-1501