Administration of cannabinoids has been linked to changes in ingestive behaviours in rodents, in the form of hyperphagia or hypophagia. These effects have been attributed to the action of cannabinoid 1 receptors (CB1R) present in both the central nervous system and the digestive tract. While studies have observed that CB1R agonists and antagonist increase and decrease gross food intake respectively, the role of CB1R in other eating behaviours, such as food preference, is largely unknown. In two separate experiments, we tested the effects of stimulation of the CB1R located in two brain regions (the lateral hypothalamus [LH] and the nucleus accumbens shell [NAs]) on food intake and food preference. Adolescent male and female rats (n = 8 per group) received 5 injections of the CB1R agonist WIN 55, 212-2 (WIN 55) or of the vehicle solution in one of these brain regions. Nine days following the last injection, the rats' food preference was assessed using a modified version of the Behavioural Satiety Sequence (BSS) and a food choice test (FC), in which they had access to regular rat chow and two palatable (high in sugar or high in fat) diets. In both experiments, WIN 55-treated female rats displayed a tendency to preferentially eat the high fat diet compared to controls. In contrast, only the male rats that received WIN 55 in the LH had a tendency to favor the high fat food, compared to the controls. Our findings support endocannabinoids to exert sex- and region-specific effects on food preference.