Spatial navigation impairments are among the earliest clinical manifestations of Alzheimer’s Disease (AD). Pyramidal cells of the hippocampus fire selectively when the animal is in a specific location in the environment, leading to the theory that the hippocampus plays a crucial role in forming a cognitive map of the environment. The phenomenon of “remapping”, where specific cells exhibit selective firing in distinct environments, is thought to support the formation of different memories. By performing in-vivo electrophysiological recordings in freely moving mice while they navigate through different environments, we characterized 1) if the remapping of different hippocampal place cells (CA1, CA3, DG) is altered in the APP/PS1 mouse model of AD, a model known for spatial navigation deficits; 2) the potential involvement of CA3 interneurons in the early hyperexcitability of the CA3 network, a feature shared by both AD patients and the APP/PS1 mouse model. While interneuron firing rates and place cell remapping are mostly maintained in the CA1 cells during the early phases of plaque deposition, we found several alterations that are present in the CA3 cells. By investigating the interplay between CA3 place cell remapping and the role of interneurons, our research contributes to a deeper understanding of the neurophysiological changes associated with spatial navigation impairments in AD. This knowledge may pave the way for novel therapeutic approaches targeting specific alterations in the hippocampal network.