Spinocerebellar ataxia (SCA) is a rare neurodegenerative disorder characterized by cerebellar dysfunction, involving Purkinje cell (PC) degeneration and cerebellar atrophy. With over 40 genetic variations, our focus is on SCA14 which is caused by mutations in the Protein Kinase C Gamma Gene (PRKCG). In our laboratory, we are taking advantage of two mouse models (PKCγ-S361G transgenic line and PKCγ-A24E knock-in line) with a constitutively active PKCγ, showing PC dendritic disruption and an ataxic motor phenotype. Using RNA-sequencing data comparing cerebellar samples from PKCγ-A24E mice with WT mice, we found that the Progestin and AdipoQ Receptor member 7 (PAQR7) had a reduced mRNA expression in the cerebellum of PKCγ-A24E mice making it a potential mediator of the effects of PKCγ in Purkinje cell dendritic development. This study aims to investigate a potential role of PAQR7 for Purkinje cell dendritic development. The function and the involvement of PAQR7 in Purkinje cell dendritic development is currently being studied in organotypic slice cultures in which Purkinje cell dendritic arbors develop during the culture period. We are using the specific Progesterone agonist Org OD 02-0 to manipulate PAQR7 activity and assess the effects of receptor activation for Purkinje cell dendritic development. We will also compare slice cultures from SCA14 mouse models with WTs, followed by morphological analysis of PC dendritic arbors. The results of our current analysis will be presented at the meeting with the aim to better characterize the potential role of PAQR7 for Purkinje cell dendritic development.