GABAA receptors modulate anxiety-like behavior through the central amygdala area in rats with higher physical activity

In this study, our objective was to explore the role of GABAA receptors within the amygdala region in modulating anxiety-like behavior in response to voluntary physical activity. We employed a stereotaxic instrument to implant two cannulae in the amygdala area, through which we administered the GABAA receptor antagonist, bicuculline. Subsequently, we exposed the animals to a running wheel for a duration of three hours over four weeks. Following this exercise regimen, we conducted behavioral tests, including the open field and elevated plus maze tests, to assess anxiety-like behavior. The findings revealed that the blockade of GABAA receptors led to reduced physical activity, subsequently resulting in increased anxiety-like behavior in rats.