In this study, our objective was to explore the role of GABAA receptors within the amygdala region in modulating anxiety-like behavior in response to voluntary physical activity. We employed a stereotaxic instrument to implant two cannulae in the amygdala area, through which we administered the GABAA receptor antagonist, bicuculline. Subsequently, we exposed the animals to a running wheel for a duration of three hours over four weeks. Following this exercise regimen, we conducted behavioral tests, including the open field and elevated plus maze tests, to assess anxiety-like behavior. The findings revealed that the blockade of GABAA receptors led to reduced physical activity, subsequently resulting in increased anxiety-like behavior in rats.