The entopeduncular nucleus EP, one of the basal ganglia(BGs), is considered one of the essential output nuclei, sending synapses belonging to the pathways essential for normal motor activity to two specific regions in the ventral lateral thalamic nuclei(VL) and ventral anterior thalamic nuclei(VA).The basal ganglia innervate the thalamus through 3 pathways:direct, indirect, and hyper-direct.By these three pathways, we get an innervation that causes disinhibition or inhibition of the thalamus.The research aimed to study the thalamus, the relay center for transmitting information received from most nuclei in the brain to the cortex.Our work has focused on the characterization and learning of the synapse and the GABAergic projection between the EP and the thalamus.We identified the two regions and checked the quality of the cells.At first, we worked on finding the proper slicing method for the brain to get the two regions in one brain slice to ensure the presence of our synapse for further work.We then worked with Short-Term Plasticity (STP) protocols to record the response of cells in the thalamus as a result of EP stimulation. According to many studies, it is known that there is a high dispersion of CB1Rs in the basal ganglia, especially in the striatum, EP, GP, and SNr, and that Ep neurons express dopamine receptors.Our last objective was to examine the effect of dopamine modulation on short-term synaptic plasticity in the thalamus by adding D1 and D2-type dopamine receptor blockers. After the analysis, we detect a difference indicating the effect of blocking D1R and D2R.