Early life stress (ELS) due to neglect or abuse, has profound and lasting effects on adult neural function, contributing to various psychopathologies. Utilizing the well-established model of early life maternal separation (ELMS), this study investigated the impact of ELS on adult behavior. For the maternally separated (MS+) group, pups were separated from their mother for 3 hours from postnatal day 1 to 14, while control (stress naive) group pups remained in their home cages. Behavioral assessments from week 8-10 (young adults) revealed significant differences between MS+ and control mice. Both male and female MS+ mice exhibited heightened exploratory behavior and anxiety levels compared to controls. Additionally, male MS+ mice displayed reduced working memory, while female MS+ mice exhibited significantly impaired spatial memory. Social preference was diminished in both sexes of MS+ mice. Evaluation of fear memory indicated decreased contextual and cued learning in male MS+ mice compared to controls, indicating a plausible impairment in the recall of fear memory. These findings highlight the behavioral consequences of early life stress, with MS+ mice demonstrating altered behavior across multiple domains. Moreover, the effects differ in some instances between the sexes. Our findings underscore the importance of early life experiences in shaping adult behavior and emphasize the need for further research to elucidate the underlying neurobiological mechanisms. These studies are crucial for developing targeted interventions to mitigate the long-term effects of early life adversity.