Behavioural states modulate neural activity in cortical and subcortical sensory areas as early as in the superior colliculus (SC) and the retina. To better understand the purpose of behavioural modulation at these early stages of visual processing, we investigated how locomotion affects various visual tuning properties in distinct functional cell types of the mouse retina and SC. We employed two-photon imaging of calcium activity in retinal axon terminals and neurons of the superficial SC (sSC) while mice were head-fixed and able to run at will on a treadmill. Neural activity was recorded in response to drifting sinusoidal gratings, which varied in their temporal and spatial frequency, direction of motion, and contrast. Behaviour of each trial was classified as stationary or running (running speed above or below 0.5 cm/s). We replicated previous findings that running affects the gain and offset of direction tuning curves of about half the retinal axons and neurons in the sSC. Intriguingly, running did not simply show linear effects on temporal and spatial frequency tuning but increased preferred temporal frequencies while decreasing preferred spatial frequencies in more than 50% of sSC neurons. Tuning of retinal axons followed the same trend albeit in a smaller population (10-30%). To functionally classify neurons and axon terminals, we also presented full-field contrast chirps and stationary circles of different sizes. We are currently analysing these data to relate cell function to behavioural modulation. Together, these findings suggest that early stages of visual processing adapt to the environmental statistics caused by locomotion.