Locomotion is an essential and conserved movement that allows humans and animals to interact with their surroundings. Although locomotion appears seemingly effortless, it is an intricate motor behavior that requires the orchestration of several supraspinal and spinal neuronal substrates activating many axial and limb muscles. Thus enabling organisms to navigate through and adapt to their environment. Such context-dependent and episodic locomotor behaviors require an interruption or arrest to adjust the movement toward the aimed goal. However, the neural pathways of motor arrest behaviors upon salient environmental changes and aside from fear-related and defensive contexts, are poorly understood. Recently, Goñi-Erro H. et al., Nat Neurosci, 2023 discovered a population of glutamatergic neurons in the rostral pedunculopontine nucleus (PPN) expressing Chx10 evoking a unique whole-body motor arrest as well as apnea and bradycardia. However, the distinct input and output connectivity of Chx10-PPN neurons and the functional involvement of other brain areas in the described behavior are mainly unknown due to methodical difficulties allowing their discrete identification. To elucidate the presynaptic input neurons of Chx10-PPN neurons, we used recent Rabies virus-based monosynaptic tracing tools allowing consistent labeling of input neurons and assigned them to distinct brain regions utilizing the Allen Mouse Brain CCF. Second, we combined viral tracing from Chx-10 PPN neurons and in-situ hybridization to investigate the transmitter phenotype of distinct postsynaptic targets in the Medulla. Together, this work will provide insights into the neuronal mechanism of context-dependent selection of arresting motor behaviors and link those motor outputs to cellular activities.