Posttraumatic stress disorder (PTSD), a psychopathological condition triggered by traumatic events, affects the brain's energy demand. The median prefrontal cortex (m-PFC) plays a crucial role in regulating the fear response. Moreover, it contains glucose-sensing glucose transporter 2 (GLUT2)-containing neurons. Therefore, we hypothesized that manipulation of these mPFC-GLUT2 cells could impact the behavioral outcome.An adeno-associated viral vector was used to deliver a stimulatory DREADD sequence into the mPFC of mice from both sexes. The infected cells were stimulated by clozapine-N-oxide right after a foot shock trauma. In addition, at the same time half of the animals got 16% sucrose solution to drink for 24h. We investigated the development of acute stress disorder-like symptom (ASD) at 24 hours and PTSD-like freezing behavior 14 days after the trauma. Immunohistochemistry confirmed the correctness of the injection.The amount of sucrose consumed by the mice was higher than that of. However, the increase in weight remained constant across all groups during the whole experiment. Overall, females appeared to be more affected than males. However, contrary to what we anticipated, the stimulation of GLUT2 positive cells in the PFC and post-trauma sucrose drinking did not result in a synergistic effect on freezing behavior. This was observed in both a contextual and cue-dependent way.Our failed attempt to confirm the role of mPFC-GLUT2 cells in the development of PTSD-like behaviour suggest that other brain areas might be involved. Given their enhanced vulnerability more attention should be paid to research with females.