Dendrites play a fundamental role in neuronal computation. It is thought that functionally defined regions of dendritic branches, termed ‘dendritic computational units’ are used to decode information transmitted by synaptic input. This information is encoded by the corresponding dendritic output, to perform complex neuronal computations. To investigate dendric input-output in vivo, it is necessary to record synaptic input and dendritic output simultaneously. To do this we perform simultaneous glutamate imaging and voltage imaging from the spiny dendrites of single Purkinje neurons in awake mice. Single cerebellar Purkinje neurons were labelled with a voltage sensitive dye (ANNINE-6plus; Roome and Kuhn, 2018) and the genetically encoded glutamate sensor; iGluSnFR3, using a chronic cranial window with access port (Roome and Kuhn, 2014). Different modes of sensory stimuli were used to evoke excitatory synaptic input to Purkinje neuron dendrites. Stimuli included a light, a sound, and an air puff directed towards the whiskers. Functional imaging was performed during sensory stimulation and the corresponding dendric input-output signals were imaged from Purkinje neuron dendrites using two-photon microscopy (box scans at 565Hz or 61Hz). Synaptic input from climbing fibers and parallel fibers were evoked by sensory stimulation and we investigate the integration of these inputs at single dendritic branches of the Purkinje neuron.