Lindane, gamma hexachlorocyclohexane is organochlorine compound that is environmental pollutant and is used as a pesticide and scabicide even today in a number of undeveloped countries. Lindane induces seizures refractory to conventional antiepileptics. Delta sleep-inducing peptide (DSIP) is an endogenous peptide that is constantly present in several different brain regions showing neuroprotective The aim of our study was to examine the effects of DSIP supplementation on electroencephalographic (EEG) ictal activity induced by lindane in rats. Adult male rats with previously implanted electrodes for EEG registration were treated for one week by DSIP (0.5 mg/kg, i.p.) or dimethyl-sulfoxide (DMSO, 0.5 ml/kg) daily. Lindane (8 mg/kg, i.p.) was thereafter injected and ictal activity recorded. Number and duration of EEG ictal periods were analyzed, as well as spectra characteristic by wavelet and fast Fourier transformation. Number of lindane-induced EEG ictal periods in DSIP-supplemented animals were significantly decreased, but its duration was unaltered compared to the vehicle – treated group. DSIP-supplemented animals showed significantly altered spectral characteristics of lindane-induced ictal EEG activity when compared to the vehicle – treated group (i.e. reduced total spectral power). It could be concluded that DSIP supplementation ameliorates lindane toxic effects manisted in EEG as ictal activity. Financed by FA4Lin Project (Serbian Ministry of Science and TUBITAK).