The eyes are highly mobile organs, and their direction and position rely on visual and proprioceptive information. Surprisingly, classical proprioceptors (muscle spindles and Golgi tendon organs) are absent in the extraocular muscles (EOMs) of most mammals, except for even-toed ungulates (e.g. pigs). Instead, most mammals have a structure called palisade endings located at the EOM myotendinous junction. Their localization and similarity with Golgi tendon organs suggest they could be sensory structures providing eye position information. However, palisade endings are cholinergic, and their cell bodies are in the oculomotor nuclei. Today the function of palisade endings is still debated. This work aimed to compare the molecular phenotype of classical proprioceptors and palisade endings in pig EOMs. This study used EOMs from pigs due to the presence of both classical proprioceptors and palisade endings. Thus, 10-µm thick sections and whole-mount preparations from EOMs were fluorescently labeled with various antibodies and toxins (neurofilament, synaptophysin, choline acetyltransferase [ChAT], vesicular glutamate transporter 1 [VGLUT1], phalloidin, and α-bungarotoxin [BTX]). The molecular findings indicate that VGLUT1 is expressed in Golgi tendon organs and muscle spindles, while palisade endings lack VGLUT1. Furthermore, palisade endings exhibit ChAT-immunoreactivity, whereas sensory nerve terminals in muscle spindles and Golgi tendon organs lack ChAT expression. Only motor terminals in the polar region of muscle spindles express ChAT. BTX, a marker for motor terminals is only present in the polar region of muscle spindles. Our results reveal that palisade endings and classical proprioceptors in pig EOMs exhibit distinct molecular characteristics.