In this study, we aimed to elucidate the anatomical and ultrastructural characteristics of CNS border-associated macrophages (BAMs) in the context of ischemic stroke. At 7 days after ischemia, the number of BAMs, identified by the expression of CD206, Lyve1, and Iba1, increased compared to the contralateral cortex. They were primarily distributed along the meninges and PVS within the lesion core, while their presence within the extravascular parenchyma was limited. Notably, a subset of BAMs did not express Lyve1 in the meninges and PVS. The heterogeneity of BAMs based on their morphology and marker expression was revealed by immunohistochemistry using CD206, Lyve1, and platelet-derived growth factor receptor beta (PDGFR-β), a marker previously identified for leptomeningeal and perivascular fibroblasts. Meningeal BAMs, intermingling with PDGFR-β+ fibroblasts, exhibited an amoeboid morphology and were CD206+Lyve1+. In contrast, BAMs located in the subpial space, characterized by a thin cell body and slender processes with partial contact with PDGFR-β+ fibroblasts, were Lyve1-. BAMs in the PVS, surrounded by PDGFR-β+ perivascular fibroblasts, were Lyve1- and displayed an elongated, stretched morphology with slender processes. BAMs exposed to the extravascular parenchyma exhibited variable morphologies and were CD206+Lyve1+. These findings were further supported by immuno-electron microscopy and correlative light- and electron-microscopy. CD206+ macrophages were found both inside and outside perivascular PDGFR-β+ fibroblasts, whereas Lyve1+ macrophages were only detected outside PDGFR-β+ fibroblasts. Collectively, our findings suggest distinct morphological characteristics and spatial dynamics of BAMs in response to ischemic stroke.