The gestational gut microbiota exerts significant influence on infant neurobehavior and postnatal brain resilience. This study addresses challenges encountered in assessing the effects of gestational antibiotic administration (GAA) and perinatal asphyxia (PA) on offspring neurodevelopmental reflexes.A preliminary study evaluated the effects of an initial antimicrobial cocktail (GAA-1) and PA on Wistar rat offspring. GAA-1 was initiated on gestational day 11 using a cocktail comprising ampicillin, vancomycin, neomycin, clindamycin, and amphotericin-B, followed by PA exposure on postnatal day (PND) 6. Early-life behavioral tests, including righting reflex (RR), limb grasping reflex (LGR), negative geotactic reaction (NGR) and grip strength response (GSR), were conducted on PND7-9.Due to elevated miscarriage rates, the cocktail was revised to a pregnancy-safe combination (GAA-2) comprising ampicillin, vancomycin, neomycin, and meropenem. A multi-strain probiotic was introduced alongside the antibiotic cocktail to investigate its impact on offspring neural development. Neurodevelopmental reflexes were repeated on PND7-9.GAA-1 led to increased miscarriages and reduced offspring birth weight. PA resulted in decreased oxygen saturation levels (45.41%). Response time was consistently prolonged in both PA and antibiotic groups, with minor variations across tests.GAA-2 did not induce miscarriages. Offspring from the antibiotic group exhibited impaired neuroreflexes in RR and NGR, partially mitigated by probiotic supplementation. No significant effect was observed in GSR.Preliminary findings suggest potential early-onset brain impairment due to maternal gut disturbance and PA. Probiotics show promise in offering protective effects against PA-related neurodevelopmental deficits. Further exploration of maternal microbiota's role in brain development may yield novel therapeutic approaches.