The arcuate nucleus (ARC) is a significant brain region involved in energy homeostasis, specifically feeding behaviour. It is widely reported that nicotine consumption reduces feeding behaviour and body weight in both humans and rodents. Milleu et al. (2011) demonstrated that nicotine increases the activity of the Pro-opiomelanocortin (POMC) positive cells in this nucleus. Huang et al. (2011) reported that nicotine increases the activity of POMC cells and NPY-positive cells. This finding may seem contradictory as NPY neurons promote hunger signals while POMC neurons generate signals of satiety. Previous research has shown that cells respond differently to glucose concentrations in electrophysiology recordings (Garduño et al., 2019). In this study, we conducted electrophysiology and calcium imaging recordings in the ARC nucleus of brain slices from male Wistar rats at physiological glucose concentrations (2mM) in the presence of nicotine. Our findings indicate that nicotine reduces the firing frequency of NPY neurons by increasing GABAergic sIPSCs. Furthermore, nicotine enhances the activity of POMC cells by increasing glutamatergic sEPSCs. Calcium imaging recordings supported these results. Cells identified as NPY decreased the global activity of the nucleus, while POMC increased activity in the presence of nicotine. These findings may account for the behavioural effects associated with nicotine consumption and the reduction in body weight.