The noradrenergic (NA) system plays a critical role in memory consolidation, including declarative memory. Recognition memory, an important aspect of declarative memory, involves the ability to acquire and recall information to identify familiar stimulus. Novel Object Recognition (NOR) is a widely used task to assess this type of memory in rodents. This test is based on the innate preference for novelty and the ability to discriminate novel and familiar objects. Post-training yohimbine administration, an a2-adrenoceptor antagonist, enhances object location memory in mice, suggesting that increases in NA signaling are associated to an enhancement in memory consolidation. Atomoxetine (ATO; a NA reuptake inhibitor) improves impaired NOR in a mouse model of hyperactivity disorder. Despite this evidence using post-training manipulations, the involvement of the NA system in the acquisition of NOR is unknown. The aim of the present study was to evaluate the modulation of the NA system in the acquisition of NOR in male and female Swiss mice. First, ATO (0 or 3 mg/kg) was injected 30 min before the training session. Second, following the same schedule, ICI 118-551 (0 or 10 mg/kg), a NA β2 receptor antagonist, was injected before the training session. Long-term memory was assesed 48h (study 1) and 24h (study 2) later. The NA reuptake inhibitor ATO facilitated object recognition in females, but not in males. ICI 118-551, prevented memory recognition in both, male and female mice. Our findings suggest that the NA system, via β2 receptors, may play a role in the acquisition of recognition memories.