Omentin-1 (OMNT1), is adipokine involved in regulation of energy metabolism, insulin sensitivity, and reproduction. Our previous study documented expression and in vitro effect of OMNT1 on endocrine function of porcine anterior pituitary cells. However, its role in hypothalamus remains unknown. The present study examines expression and impact of OMNT1 on the mouse hypothalamic neuronal GT1-7 cells (in vitro model of GnRH-secreting neurons). The mRNA expression of OMNT1 and its potential receptor, insulin receptor, as well as co-localization of OMNT1 with GnRH, were determined using RT-qPCR and immunocytochemistry. GT1-7 cells were treated with OMNT1 at increasing doses of 10, 50, and 100 ng/ml. GnRH levels (15 and 30 min) were measured using ELISA, while Gnrh1 (15, 30min, 24h), Caspase-3, Bax, and Bcl2 (24h) expression was analyzed by RT-qPCR. Also, alamarBlue assay assessed OMNT1's impact on cell viability. Statistical analysis involved Student's t-test (n=4, p<0.05). The results showed OMNT1 mRNA expression and co-localization with GnRH in GT1-7 cells. Also, OMNT1 enhanced Gnrh1 transcript levels at 100 ng/ml after 30 min of incubation and had modulatory effect on GnRH secretion in GT1-7. Interestingly, OMNT1 at 50 and 100 ng/ml increased neuronal cell viability and ratio of anti-apoptotic Bcl2 to pro-apoptotic Bax, especially at 100 ng/ml. In conclusion, our findings demonstrate for the first time OMNT1 expression in mouse hypothalamic neuronal cells and its important role in the in vitro regulation of GnRH secretion and apoptosis in GT1-7. Further study is required to validate in vivo role of hypothalamic OMNT1 in the reproductive effects.