The gut microbiota plays a crucial role in maintaining the physical and mental well-being of the host. It is influenced by diverse factors, including pesticides. Glyphosate, a globally used herbicide, is understood to be harmless to humans as it targets the shikimate pathway, which is absent in animal cells. However, this idea is now being challenged as research suggests that glyphosate may negatively affect the pathway in microorganisms residing in the gut, such as gut bacteria, which could have an impact on the host. Former research has tested either high dose exposure for toxicological effects or commercially available glyphosate-based herbicides in animals. In fact, the impact of glyphosate exposure at doses relevant to human health indicators, such as Acceptable Daily Intake (ADI), are currently lacking. In this study, adult C57BL/6 mice were exposed to glyphosate (0, 0.5[ADI in Europe]), 5, 50 mg/kg/day) via drinking water chronically. Behaviours were assessed for anxiety, stress-coping, cognition, and sociability. Interestingly, glyphosate exposure increased anxiety in the open field test and diminished social novelty preference in the three-chamber test in males exposed to glyphosate. In contrast, female glyphosate exposed mice had limited alterations with the only change recorded being a reduction in locomotion in open-field test. Further analysis is warranted to understand the potential mechanisms underlying these behavioural impairments. To achieve this, we aim to analyse compositional and functional changes in the gut microbiome from glyphosate exposure. Additionally, changes in the gut and brain will be explored with a focus on neuroplasticity and physiology.