Parkinson's disease (PD) is the second most common neurodegenerative disease, manifested by the loss of dopaminergic neurons. It has been shown that oxidative stress and apoptosis play a major role in the pathology of PD. One of the most commonly used models for in vitro investigation of PD is the SH-SY5Y neuroblastoma cell line and application of hydroxydopamine (6-OHDA). The 6-OHDA exerts its cytotoxicity by generating reactive oxygen species (ROS) such as hydrogen peroxide, superoxide anion and hydroxyl-radical, leading to oxidative stress by disrupting the mitochondrial membrane integrity, causing the release of cytochrome c, thus resulting in cell apoptosis. Former research of our group showed that transcranial magnetic stimulation decreased oxidative stress parameters in the 6-OHDA-induced PD model in vivo. Accordingly, the next steps aimed to provide better understanding of the action of magnetic stimulation (MS) at the cellular level. In this study we used an in vitro model of PD: the 6-OHDA treatment of retinoic acid and phorbol myristate acetate differentiated SH-SY5Y cells, as this differentiation strategy potentiates the dopaminergic phenotype. The differentiation of the neuron-like cells was confirmed by the analysis of neuronal markers by immunocytochemistry, Western blot and HPLC method, while the electrophysiologic functionality was determined by Ca2+-imaging and patch clamping. The protective effects of MS were examined by analyzing oxidative stress and antioxidant protection parameters, cell survival, as well as the preservation of electrophysiological characteristics.Acknowledgement: Ministry of Science, Technological Development and Innovation RS 451-03-66/2024-03/ 200178