Intracellular membrane protein trafficking is crucial for both normal cellular physiology and cell-cell communication. It is now established that intracellular trafficking not only uses the conventional secretory pathway but also an alternative secretion pathway able to bypass the need for passage through the Golgi apparatus, which is collectively termed unconventional protein secretion. One of the major actors of this pathway is the vesicular SNARE VAMP7, which is involved in the secretion of extracellular vesicles of late endosomal origin called exosomes. It was recently shown that exosomes can carry misfolded alpha-synuclein and that exosomes purified from patient blood could induce Parkinsonism in healthy mice. In this project, we aim to characterize the role of VAMP7 in exosome secretion and uncover its contribution to neurodegeneration in Parkinson's disease. Using cultured cells and in vivo models, we characterize the VAMP7-dependent secretome released by neurons and glial cells. We will assess the effects of these cellular secretomes on neuronal morphology, viability, and function. Behavioral studies and histological analysis in mutant mouse models will allow us to shed light on the role of late endosomal autophagic secretion in neurodegenerative processes, providing insights into Parkinson's disease pathogenesis.