Spinal cord injury is associated with axonal damage and degeneration, leading to the dysfunction of neuronal networks and hence, severe impairment of neurological function. Injured neurons response depends both on extracellular cues (mainly non-permissive, such as myelin debris, extracellular matrix protein such as CSPG, glial and fibrotic scar, inflammatory cues, …) and their intrinsic capacity. How microtubule-associated protein (MAP) contribute to this response is the objective of the present work. MAP6 is the first neuronal microtubule (MT) inner protein discovered and preliminary results show that MAP6 inside MT induces a very high stability, associated with a helical shape and persistent holes in MT lattices. Thus, MAP6 could contribute to neuronal stability and resistance of axons to external constraints. Therefore, we are investigating the function of MAP6 in axonal regeneration, by using map6-ko mice. With a specific microfluidic device that we have designed, oriented cortical embryonic neurons are axotomized by a compressive force. Axonal regeneration within the first 96 hours post-injury is under investigation to determine MAP6 involvement in axon regeneration. In parallel, we are studying the function of MAP6 in adult axonal regeneration. Thus, adult dorsal root ganglion neurons are plated on permissive or non-permissive substrates. Initiation, elongation, total neuritic length and branching are under analysis. Preliminary data suggests that MAP6 is involved at least in the initiation of adult axonal regeneration.