Chronic pain is a major disease burden with high incidence compared to other chronic diseases and with high persistence among affected individuals over many years.Satellite glial cells (SGCs) is a supportive cell type that ensheath the body of sensory neurons in the peripheral nervous system and in health SGCs are believed to contribute to neuronal homeostasis. However, evidence suggests that in certain disease states such as a traumatic nerve injury, satellite glial cells contribute to the development of chronic pain. Recent advances in single-cell RNA sequencing (scRNAseq) have prompted the search for SGC subtypes, but the validation and characterization on the topic is largely unexplored. Here we used scRNAseq to identify five subclusters among SGCs, of which four can be replicated using other published scRNAseq datasets. One subtype is characterized by the expression of markers that have traditionally been used to label SGCs in the literature. Two other subtypes differ from each other in enriched genes but have in common the lower expression of classical markers. And the fourth subtype is characterized by the expression of immune response related genes. Using replicable subtype markers (based on our own as well as other scRNAseq datasets), the SGC subtypes were characterized and validated using IHC and ISH, showing clear distinction between the new subtypes and the ones expressing classical markers. In conclusion, we visually represent the heterogeneity of SGCs to back up scRNAseq data with protein markers distinguishing different SGC subtypes, enabling further studies of concrete subtypes.