Sex and age play significant roles in spatial and contextual memory formation; however, these factors are understudied especially considering the ongoing maturation of prefrontal cortex and hippocampus through juvenility until adulthood. The receptor of Oxytocin (OXTR) has sex-dimorphic characteristics and is expressed in these regions. We previously demonstrated that blockade of prefrontal OXTR impaired extinction of contextual fear memory in juvenile females but not males (Maroun 2020). The hippocampus is central to spatial memory formation and performance in spatial memory tasks is age and sex-dependent. The present study aims to investigate sex differences in novelty-based object location memory (OLM) across development, focusing on the role of OXTR within the hippocampal CA1. We first quantified CA1 expression of c-Fos, a neuronal activation marker, after OLM in juvenile rats and showed that OLM increases the expression of CA1 c-Fos in males but not females. Additionally, local CA1 OXTR blockade during the OLM consolidation in juveniles, adolescents, and adults showed impaired long-term OLM exclusively in juvenile males without affecting juvenile females. Conversely in adults, OXTR blockade impaired OLM in females but not males, with similar outcomes observed in adolescent rats. Our investigation reveals the intricate dynamics of CA1 involvement in novelty-based OLM across different developmental stages and sex. Specifically, our findings highlight a shift in the role of oxytocin in OLM during the pre-pubertal and adulthood periods that sex-dependent. This underscores the significant roles of sex, age, and oxytocin in shaping spatial memory