The state-of-the-art techniques for all-optical interrogation of neuronal circuits in vivo typically combine two-photon (2P) calcium imaging and digital holography to precisely activate target neurons with sculpted light patterns. This approach is however limited in speed and in throughput, ultimately due to the intrinsic constraints of digital holography. We employed acousto-optic deflectors (AOD)-based 2P approaches (“ULoVE”, Villette et al. 2019; “3D-CASH”, Akemann et al. 2022) to simultaneously record and perturb neuronal activity in awake head-fixed mice across single planes or three-dimensional volumes with unprecedented speed. These techniques allow maximizing signal collection and opsin activation efficiency within a minimal dwell time, by sampling the targets neurons with extended shapes, in a sequential fashion. For optogenetics, it meant we could activate robustly ChRmine-expressing neurons with a single visit of 70µs dwell time and, consequently, obtain the co-activation of more than 10 neurons in as little as 1ms. Such results open the way to activation of large populations of neurons at high refresh rates (e.g. ~300 neurons at 50Hz, or 1000 neurons at 15Hz). Differently from digital holography approaches, scaling up the number of target neurons does not require increasing the laser power, keeping the amount of light arriving in the brain under the physiological range. Moreover, fast scanning speed permits to combine single cell resolution optogenetics with high signal-to-noise voltage imaging, to expand further the range of physiological functions that can be dissected all-optically in the intact network.