Aims: Alzheimer's disease (AD), a neurodegenerative disorder, exhibits cognitive decline via plaque formation, inflammation, cholinergic deficits, and oxidative stress. Curcumin, derived from turmeric, exhibits neuroprotective properties, potentially counteracting AD and Parkinson's disease. With anti-inflammatory, anti-oxidant, insulin-sensitizing properties, it inhibits protein aggregation. Despite the crucial role of thyroid hormones in memory, there is limited electrophysiological evidence supporting these effects.Methods: This study, involving adult Wistar rats, aimed to explore the impact of Curcumin and L-thyroxine on the hippocampus in amyloid beta peptide (25-35)–injected AD rats, addressing limited electrophysiological evidence in the literature. Rats received intracerebroventricular injections of amyloid beta peptide (25-35), underwent an open field behavioral test, and subsequently underwent electrophysiological measurements. Under urethane anesthesia, a bipolar electrode stimulated the entorhinal cortex, while a glass micropipette recorded electrical activity in the hippocampal regions.Results: The results indicated significant changes in hippocampal electrophysiological activity in rats treated with Curcumin and thyroxine, providing in vivo evidence of their effects in AD rats.Conclusions: This integrative study, encompassing behavioral and electrophysiological data, enhances our comprehension of the intricate interplay among thyroid hormones, Curcumin's protective action, and hippocampal activity. The observed electrophysiological alterations offer insights into potential mechanisms in AD-related cognitive deficits. The research was supported by the Higher Education and Science Committee of MESCS RA (Research project №22YR-1F003).