Animals are motivated to acquire knowledge. A particularly striking example is information seeking behavior: animals often seek out sensory cues that will inform them about the properties of uncertain future rewards, even when there is no way for them to use this information to influence the reward outcome, and even when this information comes at a considerable cost. Evidence from monkey electrophysiology and human fMRI studies suggests that orbitofrontal cortex and midbrain dopamine neurons represent the subjective value of knowledge during information seeking behavior. However, it remains unclear how the brain assigns value to information and how it integrates this with other incentives to drive behavior. We have therefore developed a task to test if information preferences are present in mice and study how informational value is imparted on stimuli. Mice are trained to enter a center port and receive an initial odor that instructs them to either go to an informative side port, go to an uninformative side port, or choose freely between them. The chosen side port then yields a second odor cue followed by a delayed probabilistic water reward. The informative port’s odor cue indicates whether the upcoming reward will be big or small. The uninformative port’s odor cue is uncorrelated with the trial outcome. Crucially, the two ports only differ in their odor cues, not in their water value since both offer identical probabilities of big and small rewards. We find that mice prefer the informative port. This preference is evident as a higher percentage choice of the informative port when given a free choice (67% +/- 1.7%, n = 14, p < 0.03), as well as by faster reaction times when instructed to go to the informative port (544ms +/- 21ms vs 795ms +/- 21ms, n = 14, p < 0.001). The preference for information is robust to within-animal reversals of informative and uninformative port locations, and, moreover, mice are willing to pay for information by choosing the informative port even if its reward amount is reduced to be substantially lower than the uninformative port. These behavioral observations suggest that odor stimuli are imparted with informational value as mice learn the information seeking task. We are currently imaging neural activity in orbitofrontal cortex with microendoscopes to identify changes in neural activity that may reflect value associated with the acquisition of knowledge.