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Lab Chip Diagnostic Tools

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Seminar✓ Recording AvailablePhysics of Life

Lab-on-a-chip and diagnostic tools for COVID-19

Connie B. Chang

Prof

Montana State University

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Tuesday, October 27, 2020

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Tuesday, October 27, 2020

3:00 PM America/New_York

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Host: NYU Soft Matter Seminar

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Abstract

The SARS-CoV-2 virus has rapidly evolved into a pandemic that is threatening public health, economics, and quality of life worldwide. The gold-standard for testing individuals for COVID-19 is using traditional RT-qPCR, which is expensive and can take up to several hours. Expanding surveillance across a global scale will call for new strategies and tests that are inexpensive, require minimal reagents, decrease assay time, and allow for simple point-of-care (POC) monitoring without need of trained personnel and with quick turnaround time. To expand the speed of COVID-19 surveillance, we are working on a point-of-care microfluidic chip to enable significantly faster and easier testing. This is based upon digital drop loop-mediated isothermal amplification that will allow for rapid testing of large populations at a reasonable cost. The device will employ a nucleic-acid based test called reverse transcriptase LAMP (RT- LAMP) that operates at a temperature of 60-65°C. RT-LAMP removes the bottleneck of thermal cycling and high temperatures required by traditional RT-qPCR thermocycling. The simplicity, speed, and sensitivity will enable early treatment and response to infection.

Topics

COVID-19RT-qPCRSARS-CoV-2assay timedigital drop LAMPmicrofluidic chipnucleic-acid based testpoint-of-carereverse transcriptase LAMPsoft matter

About the Speaker

Connie B. Chang

Prof

Montana State University

Contact & Resources

Personal Website

www.chbe.montana.edu/directory/1688278/connie-chang

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