Freely Moving Mice
freely moving mice
An open-source miniature two-photon microscope for large-scale calcium imaging in freely moving mice
Due to the unsuitability of benchtop imaging for tasks that require unrestrained movement, investigators have tried, for almost two decades, to develop miniature 2P microscopes-2P miniscopes–that can be carried on the head of freely moving animals. In this talk, I would first briefly review the development history of this technique, and then report our latest progress on developing the new generation of 2P miniscopes, MINI2P, that overcomes the limits of previous versions by both meeting requirements for fatigue-free exploratory behavior during extended recording periods and satisfying demands for further increasing the cell yield by an order of magnitude, to thousands of neurons. The performance and reliability of MINI2P are validated by recordings of spatially tuned neurons in three brain regions and in three behavioral assays. All information about MINI2P is open access, with instruction videos, code, and manuals on public repositories, and workshops will be organized to help new users getting started. MINI2P permits large-scale and high-resolution calcium imaging in freely-moving mice, and opens the door to investigating brain functions during unconstrained natural behaviors.
Joint coding of visual input and eye/head position in V1 of freely moving mice
COSYNE 2022
Joint coding of visual input and eye/head position in V1 of freely moving mice
COSYNE 2022
Retinal scene statistics for freely moving mice
COSYNE 2023
Deep cortical layers encode sustained changes in light conditions in freely moving mice
FENS Forum 2024
Disruption of climbing behavior by harmaline in marker-based 3D motion capture of freely moving mice
FENS Forum 2024
Dissecting prefrontal contributions to behavioral flexibility in freely moving mice
FENS Forum 2024
Integration of autonomic readouts to study neural emotional states in freely moving mice
FENS Forum 2024
Longitudinal tracking of hippocampal activity with the InSplorer endoscope in freely moving mice
FENS Forum 2024
Nasal pressure dynamics in freely moving mice reveal modulation of the hippocampal and piriform networks by respiration
FENS Forum 2024
Neural correlates of social choices during decision-making in freely moving mice
FENS Forum 2024
Precise spatiotemporal photorelease of a systemically administered caged nicotine agonist in freely moving mice
FENS Forum 2024
Simultaneous calcium imaging and extracellular electrophysiology using CMOS-based imaging devices with an integrated carbon electrode for freely moving mice experiments
FENS Forum 2024
Spatial representation in the visual cortex, retrosplenial cortex, and (para)hippocampal formation of freely moving mice
FENS Forum 2024
Tri-dimensional kinematics of locomotor steering strategies in freely moving mice
FENS Forum 2024