Exposure to sensory regularities in the environment induces the human brain to form expectations about incoming stimuli and remains partially preserved in the absence of consciousness (i.e. coma and sleep). While regularity often refers to stimuli presented at a fixed pace, we recently explored whether auditory prediction extends to pseudo-regular sequences where sensory prediction is induced by locking sound onsets to heartbeat signals and whether it can occur across vigilance states. In a series of experiments in healthy volunteers, we found neural and cardiac evidence of auditory prediction during heartbeat-based auditory regularities in wakefulness and N2 sleep. This process could represent an important mechanism for detecting unexpected stimuli in the environment even in states of limited conscious and attentional resources.