Cookies
We use essential cookies to run the site. Analytics cookies are optional and help us improve World Wide. Learn more.
PhD
Assistant Professor, Department of Computational and Systems Biology
Showing your local timezone
Schedule
Friday, September 20, 2024
4:00 AM America/Santiago
Domain
Open SourceHost
LIBRE hub Seminar
Duration
60 minutes
Embryos issue instructions to their cells in the form of patterns of signaling activity. Within these patterns, the distribution of signaling in time and space directs the fate of embryonic cells. Tools to perturb developmental signaling with high resolution in space and time can help reveal how these patterns are decoded to make appropriate fate decisions. In this talk, I will present new optogenetic reagents and an experimental pipeline for creating designer Nodal signaling patterns in live zebrafish embryos. Our improved optoNodal reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range. We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression. Using this system, we demonstrate that patterned Nodal activation can initiate specification and internalization movements of endodermal precursors. Further, we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects. This study establishes an experimental toolkit for systematic exploration of Nodal signaling patterns in live embryos.
Nathan Lord
PhD
Assistant Professor, Department of Computational and Systems Biology
open source
Advancements in imaging speed, depth and resolution have made structured illumination microscopy (SIM) an increasingly powerful optical sectioning (OS) and super-resolution (SR) technique, but these d
open source
Optical imaging is a major research tool in the basic sciences, and is the only imaging modality that routinely enables non-ionized imaging with subcellular spatial resolutions and high imaging speeds
neuro
Nervous system function relies on the polarized architecture of neurons, established by directional transport of pre- and postsynaptic cargoes. While delivery of postsynaptic components depends on the
The postdoctoral researcher will work on an interdisciplinary project at the intersection of deep learning and comparative politics. The candidate will work in the Human-Centered Machine Learning (HuM
The CSNG Lab at the Faculty of Mathematics and Physics at the Charles University is seeking a highly motivated Postdoctoral Researcher to join our team to work on a digital twin model of the visual sy
We have an open position for a postdoctoral researcher with experience in brain-computer interfacing and artificial intelligence to further advance our new class of Brain-Artificial Intelligence (BAI)
PROJECT SUMMARY / ABSTRACT Cluster headache (CH), which affects about 1 in 1,000 people, is a severe and debilitating primary headache disorder characterized by repeated attacks occurring in clusters