Investigation of neuromodulator receptors endocytosis with the pulsed pH assay
Silvia Sposini, Rim Baccouch, Marc von Zastrow, Pierre Trifilieff, Alves Isabel, Aylin Hanyaloglu, David Perrais · FENS Forum 2024
Neurons express a variety of G protein coupled receptors (GPCRs), which profoundly condition neuronal physiology by activating intracellular signalling cascades in response to extracellular signals, including neuromodulators such as noradrenaline and dopamine. One mechanism GPCR use to tune their activity is membrane trafficking. Upon ligand binding, GPCRs are endocytosed through distinct mechanisms via interaction with different proteins (e.g clathrin, endophilin). Moreover, endocytosis is affected by membrane composition. With the aim of studying endocytosis at single event level in living c
ePoster · Neuroscience