Topic: 3D electron microscopy

Focal cortical dysplasia (FCD) is a developmental disorder of the cerebral cortex and a main cause of drug-resistant epilepsy in children. A loss of the excitation and inhibition (E/I) balance has emerged as a key feature for neuronal hyperexcitability, underlying the pathogenesis of seizures. However, it remains unclear how synaptic ultrastructure is altered in the human patients with FCD. Here we performed morphological analyses on the synaptic density, shape, and distribution of intrasynaptic organelles in the temporal cortical layer III of a FCD patient using 3D electron microscopy. Our qu