Engineering capsid-variant AAVs for selective gene delivery to dentate gyrus granule cells in the hippocampus
Severine Deforges, Jihad El Abdari, Claire Domenger, Dirk Grimm, Christophe Mulle · FENS Forum 2024
In order to interrogate the connectivity and function of local hippocampal circuits, there is a strong need to develop viral gene transfer tools which selectively target distinct neuronal populations. Here we have developed a new recombinant-capsid AAV variants (rAAV) which can infect Dentate granule cells (DGC) with high efficiency and very good selectivity.We have used an AAV-peptide library in which each rAAV displays a random 9 amino acids peptide inserted into exposed loops of the AAV9 capsid. The library was injected in vivo by steretaxy in the mouse DG. After 3 rounds of in vivo selecti
ePoster · Neuroscience